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ZEB1 antibody [N2C1], Internal

Catalog Number: GRP38

Product Overview
Product Name ZEB1 antibody [N2C1], Internal
Catalog Number GRP38
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ChIP, ICC, IF, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.1 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P37275
Entrez 6935
Product Description

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Cross-linked ChIP was performed with HeLa chromatin extract and 5 ?g of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GRP490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (GRP490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GRP490) diluted at 1:500.Red:
ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ?g 293T whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ?g of ZEB1 antibod
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