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Product Overview
| Product Name | VCP antibody |
|---|---|
| Catalog Number | GRP100 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P55072 |
| Entrez | 7415 |
Product Description
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
![VCP antibody detects VCP Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VCP stained by VCP antibody (GRP552) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GRP552) diluted at 1:500](https://www.grp-ak.de/media/catalog/product/v/c/vcp-antibody_grp552_ihc_2_2.jpg)
VCP antibody detects VCP protein by western blot analysis.A. 50 μg Rat brain lysate/extract7.5% SDS-PAGEVCP antibody (GRP552) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP552 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP(GRP552) antibody at 1:500 dilution.
VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: VCP protein stained by VCP antibody (GRP552) diluted at 1:500.Blue: Hoechst 33343 staining.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GRP552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VCP antibody detects VCP Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VCP stained by VCP antibody (GRP552) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GRP552) diluted at 1:500
Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 ?g of VCP antibody (GRP552).Western blot analysis was performed using VCP antibody (GRP552) diluted at 1:500.EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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