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Product Overview
| Product Name | Rb antibody |
|---|---|
| Catalog Number | GRP11 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Rb. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.56 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P06400 |
| Entrez | 5925 |
Product Description
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Rb antibody detects Rb protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Rb antibody (GRP463) dilution: 1:500.
Rb antibody detects Rb protein by western blot analysis.A. 30 μg A431 whole cell lysate/extract5% SDS-PAGERb antibody (GRP463) dilution: 1:500The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody (GRP463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rb antibody detects Rb protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody (GRP463) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:2
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody (GRP463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments.IP samples: HeLa whole cell extractA. Control with 4 ?g of preimmune Rabbit IgGB. Immunoprecipitation of retinoblastoma 1 protein by 4 ?g Rb antibody (GRP463)7.5 % SDS-PAGEThe immun
Rb antibody detects Rb protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Rb stained by Rb antibody (GRP463) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 anti
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