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Product Overview
| Product Name | Opsin 3 antibody [N1], N-term |
|---|---|
| Catalog Number | GRP122 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | IHC-Fr, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Opsin 3. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.51 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q9H1Y3 |
| Entrez | 23596 |
Product Description
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. In addition to the visual opsins, mammals possess several photoreceptive non-visual opsins that are expressed in extraocular tissues. This gene, opsin 3, is strongly expressed in brain and testis and weakly expressed in liver, placenta, heart, lung, skeletal muscle, kidney, and pancreas. The gene may also be expressed in the retina. The protein has the canonical features of a photoreceptive opsin protein. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000 |
|---|
Validation Images
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Opsin 3 antibody [N1], N-term (GRP574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/o/p/opsin-3-antibody-n1-n-term_grp574_wb_1_2.jpg)
![Opsin 3 antibody [N1], N-term detects Opsin 3 protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Opsin 3 protein stained by Opsin 3 antibody [N1], N-term (GRP574) diluted at 1:250.Red: beta Tubulin 3/ TU](https://www.grp-ak.de/media/catalog/product/o/p/opsin-3-antibody-n1-n-term_grp574_ihc_2_2.jpg)
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Opsin 3(GRP574) antibody at 1:250 dilution.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Opsin 3 antibody [N1], N-term (GRP574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Opsin 3 antibody [N1], N-term detects Opsin 3 protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Opsin 3 protein stained by Opsin 3 antibody [N1], N-term (GRP574) diluted at 1:250.Red: beta Tubulin 3/ TU
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