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Product Overview
| Product Name | MAP2 antibody |
|---|---|
| Catalog Number | GRP167 |
| Species/Host | Rabbit |
| Reactivity | Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse MAP2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.15 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P20357 |
| Entrez | 17756 |
Product Description
MAP2 antibody
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000 |
|---|
Validation Images
Mouse tissue extract (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GRP619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAP2 antibody detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MAP2 antibody (GRP619) diluted at 1:500.
MAP2 antibody detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MAP2 antibody (GRP619) diluted at 1:500.
MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: MAP2 stained by MAP2 antibody (GRP619) diluted at 1:250.Blue: Fluoroshield with DAPI.Antigen Retrieval: Citrate buffer, pH 6.0,
MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: MAP2 stained by MAP2 antibody (GRP619) diluted at 1:250.Blue: Fluoroshield with DAPI.Antigen Retrieval: Citrate buffer, pH 6.0,
MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: MAP2 stained by MAP2 antibody (GRP619) diluted at 1:250.Blue: Fluoroshield with DAPI.Antigen Retrieval: Citrate buffer, pH 6.
MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.MAP2 stained by MAP2 antibody (GRP619) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.MAP2 stained by MAP2 antibody (GRP619) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MAP2 antibody detects MAP2 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 protein stained by MAP2 antibody (GRP619) diluted at 1:500.Red: Tau,
MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis .Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Grenn: MAP2 protein stained by MAP2 antibody (GRP619) dilu
MAP2 antibody detects MAP2 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 protein stained by MAP2 antibody (GRP619) diluted at 1:500.Red: MAP2
MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: MAP2 protein stained by MAP2 antibody (GRP619) diluted at 1:250.Red: Tau, stained by Tau antibody (GRP619) diluted at 1:5
MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 stained by MAP2 antibody (GRP619) diluted a
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