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Product Overview
| Product Name | LAMP1 antibody [GT25212] |
|---|---|
| Catalog Number | GRP176 |
| Species/Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LAMP1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Purity | Affinity purified by Protein A. |
| Clone ID | GT25212 |
| Uniprot ID | P11279 |
| Entrez | 3916 |
Product Description
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was dev](https://www.grp-ak.de/media/catalog/product/l/a/lamp1-antibody-gt25212_grp628_wb_1_2.jpg)
![LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.grp-ak.de/media/catalog/product/l/a/lamp1-antibody-gt25212_grp628_ihc-p_2_2.jpg)
![LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.grp-ak.de/media/catalog/product/l/a/lamp1-antibody-gt25212_grp628_ihc-p_1_2.jpg)
![LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:2000.Red: alpha Tubulin 4a, a cytoskel](https://www.grp-ak.de/media/catalog/product/l/a/lamp1-antibody-gt25212_grp628_icc_1_2.jpg)
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was dev
LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LAMP1 stained by LAMP1 antibody [GT25212] (GRP628) diluted at 1:2000.Red: alpha Tubulin 4a, a cytoskel
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