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Product Overview
| Product Name | Homer1 antibody |
|---|---|
| Catalog Number | GRP113 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.75 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q86YM7 |
| Entrez | 9456 |
Product Description
This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GRP565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GRP565) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GRP565) antibody at 1:500 dilution.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GRP565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GRP565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Homer1 antibody detects Homer1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Homer1 protein stained by Homer1 antibody (GRP565) diluted at 1:500.Red: beta Tubu
Homer antibody detects Homer protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Homer protein stained by Homer antibody (GRP565) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained b
Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 ?g of Homer antibody (GRP565).Western blot analysis was performed using Homer antibody (GRP565).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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