Homer1 antibody

Product Overview

Product Name	Homer1 antibody
Catalog Number	GRP113
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary.
Alternative Names	(click to expand) homer scaffold protein 1 \| HOMER \| HOMER1A \| HOMER1B \| HOMER1C \| SYN47 \| Ves-1

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.75 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	Q86YM7
Entrez	9456

Product Description

Application Notes

Dilution Range	WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:100-1:500

Validation Images

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GRP565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GRP565) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GRP565) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GRP565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Homer1 antibody detects Homer1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Homer1 protein stained by Homer1 antibody (GRP565) diluted at 1:500.Red: beta Tubu

Homer antibody detects Homer protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Homer protein stained by Homer antibody (GRP565) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained b

Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 ?g of Homer antibody (GRP565).Western blot analysis was performed using Homer antibody (GRP565).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

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Homer1 antibody

Catalog Number: GRP113