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Product Overview
| Product Name | GPR30 antibody [C2C3], C-term |
|---|---|
| Catalog Number | GRP41 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR30. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.12 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q99527 |
| Entrez | 2852 |
Product Description
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GRP493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](https://www.grp-ak.de/media/catalog/product/g/p/gpr30-antibody-c2c3-c-term_grp493_wb_1_2.jpg)
Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GRP493) antibody at 1:150 dilution.
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GRP493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody
Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GRP493) antibody at 1:150 dilution.
GPR30 antibody detects GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody (GRP493) diluted at 1:500.Red: beta Tubulin
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