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Product Overview
| Product Name | ERM antibody |
|---|---|
| Catalog Number | GRP59 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.94 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P41161 |
| Entrez | 2119 |
Product Description
Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.
Application Notes
| Dilution Range | WB: 1:1000-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GRP511) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pr
ERM antibody detects ERM protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ERM protein stained by ERM antibody (GRP511) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, di
ERM antibody detects ERM protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ERM antibody (GRP511) diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ERM(GRP511) antibody at 1:100 dilution.
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