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Product Overview
| Product Name | Chromogranin C antibody |
|---|---|
| Catalog Number | GRP150 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Chromogranin C. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P13521 |
| Entrez | 7857 |
Product Description
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Chromogranin C antibody detects SCG2 protein by western blot analysis.A. 50 μg mouse brain lysate/extract7.5% SDS-PAGEChromogranin C antibody (GRP602) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Chromogranin C antibody detects SCG2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Chromogranin C antibody (GRP602) dilution: 1:500.
Chromogranin C antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Chromogranin C antibody (GRP602) dilution: 1:500.
Chromogranin C antibody detects SCG2 protein by western blot analysis.A. 50 μg rat brain lysate/extract7.5% SDS-PAGEChromogranin C antibody (GRP602) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Chromogranin C antibody detects Chromogranin C protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Chromogranin C antibody (GRP602) dilution: 1:500.
Sample (30 μg of whole cell lysate) A: IMR32 B: U87-MG 7.5% SDS PAGE GRP602 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The IHC-P analysis of Chromogranin C antibody was published by Peitsch WK and colleagues in the journal PLoS One in 2014.PMID: 24558503
The ICC/IF analysis of Chromogranin C antibody was published by Peitsch WK and colleagues in the journal PLoS One in 2014.PMID: 24558503
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