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beta Catenin antibody [N1N2-2], N-term

Catalog Number: GRP22

Product Overview

Product Name	beta Catenin antibody [N1N2-2], N-term
Catalog Number	GRP22
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat, Rabbit
Conjugation	Unconjugated
Tested applications	ChIP, FACS, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
Alternative Names	(click to expand) catenin beta 1 \| CTNNB \| EVR7 \| MRD19 \| armadillo

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P35222
Entrez	1499

Product Description

Application Notes

Dilution Range	WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:50-1:100

Validation Images

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ?g of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:500.

beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract

7.5% SDS-PAGEbeta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:50

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2],

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

Sample (50 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GRP474 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GRP474) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:10000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibo

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GRP474) diluted at 1:500.Red: alpha Tubulin antibody

Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 ?g of beta Catenin antibody (GRP474).Western blot analysis was performed using beta Catenin antibody (GRP474).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

beta Catenin antibody [N1N2-2], N-term (GRP474) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:50. Acquisition o

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