TDP43 antibody

Catalog Number: GRP141

Product Overview

Product Name	TDP43 antibody
Catalog Number	GRP141
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ChIP, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Alternative Names	(click to expand) TAR DNA binding protein \| ALS10 \| TDP-43

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	1.1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	Q13148
Entrez	23435

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:1000

Validation Images

TARDBP antibody detects TARDBP protein by western blot analysis.A. 30 μg Neuro2A whole cell lysate/extractB. 30 μg GL261 whole cell lysate/extractC. 30 μg NIH-3T3 whole cell lysate/extractD. 30 μg BCL-1 whole cell lysate/extractE. 30 μg Raw

Sample (50 μg of whole cell lysate) A: Rat brain 10% SDS PAGE GRP593 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GRP593) antibody at 1:100 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GRP593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GRP593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TDP43 antibody detects TDP43 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TDP43 stained by TDP43 antibody (GRP593) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TDP43 antibody detects TDP43 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDP43 stained by TDP43 antibody (GRP593) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, di

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 ?g of TARDBP

TDP43 antibody detects TDP43 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDP43 stained by TDP43 antibody (GRP593) diluted at 1:500.Red: Tau, stained by

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