Availability
- Request Lead Time
- In stock and ready for quick dispatch
- Usually dispatched within 5-10 working days
Product Overview
| Product Name | BRCA1 antibody [6B4] - ChIP grade |
|---|---|
| Catalog Number | GRP86 |
| Species/Host | Mouse |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ChIP, ICC, IF, IHC-P, IP, WB |
| Immunogen | BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 2.75 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Purity | Protein G purified |
| Clone ID | 6B4 |
| Uniprot ID | P38398 |
| Entrez | 672 |
Product Description
BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000 |
|---|
Validation Images
![BRCA1 antibody [6B4] (GRP538) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GRP538) wa](https://www.grp-ak.de/media/catalog/product/b/r/brca1-antibody-6b4-chip-grade_grp538_wb_2_2.jpg)
BRCA1 antibody 6B4 (GRP538) was used for western blot assay at 1:1000 antibody dilution. western blot assay was performed using MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate separated in a 5-15% gradient gel.Observed molecular weight of BRC
BRCA1 antibody 6B4 (GRP538) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room tempe
BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GRP538) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody
BRCA1 antibody 6B4 (GRP538) and BRCA1 antibody 17F8 (GRP538) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 c
BRCA1 antibody 6B4 (GRP538) and BRCA1 antibody 17F8 (GRP538) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay. Enrich
BRCA1 antibody [6B4] (GRP538) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GRP538) wa
Reviews
Write Your Own Review
