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Product Overview
| Product Name | BCL6 antibody [N2C1], Internal |
|---|---|
| Catalog Number | GRP20 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ChIP, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.78 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P41182 |
| Entrez | 604 |
Product Description
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
![BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extrac](https://www.grp-ak.de/media/catalog/product/b/c/bcl6-antibody-n2c1-internal_grp472_wb_2_2.jpg)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GRP472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/b/c/bcl6-antibody-n2c1-internal_grp472_wb_1_2.jpg)
![BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GRP472) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GRP4](https://www.grp-ak.de/media/catalog/product/b/c/bcl6-antibody-n2c1-internal_grp472_ihc_2_2.jpg)
BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extrac
Cross-linked ChIP was performed with Raji chromatin extract and 5 ?g of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6(GRP472) antibody(10 ?g/ml).
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GRP472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 ?g whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 ?g of pre-immune rabbit IgG C : Immunoprecipitation of
BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GRP472) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GRP4
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