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Product Overview
| Product Name | VIP antibody |
|---|---|
| Catalog Number | GRP157 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P01282 |
| Entrez | 7432 |
Product Description
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000 |
|---|
Validation Images
VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GRP609) at a dilution of 1:1000.
VIP antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. VIP antibody (GRP609) dilution: 1:500.
VIP antibody detects VIP protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GRP609) diluted at 1:1000.
VIP antibody detects VIP protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse thalamus.Green: VIP stained by VIP antibody (GRP609) diluted at 1:250.Blue: Fluoroshield with DAPI.
VIP antibody detects VIP protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VIP stained by VIP antibody (GRP609) diluted at 1:500.Red: beta Tubulin 3/ Tuj1,
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