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Product Overview
| Product Name | Vimentin antibody |
|---|---|
| Catalog Number | GRP13 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.39 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P08670 |
| Entrez | 7431 |
Product Description
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Application Notes
| Dilution Range | WB: 1:5000-1:50000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Vimentin antibody (GRP465) diluted at 1:500.
Vimentin antibody detects Vimentin protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. Vimentin antibody (GRP465) diluted at 1:500.
Vimentin antibody detects Vimentin protein by western blot analysis.A. 20 μg 293T whole cell lysate/extract B. 10 μg 293T whole cell lysate/extract C. 5 μg 293T whole cell lysate/extract D. 1 μg 293T whole cell lysate/extract10% SDS-PAGEViment
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Vimentin antibody (GRP465) diluted at 1:500.
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Vimentin antibody (GRP465) diluted at 1:500.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GRP465) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GRP465) diluted at 1:2000.
Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Vimentin stained by Vimentin antibody (GRP465) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Vimentin stained by Vimentin antibody (GRP465) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (GRP465) diluted at 1:500.Blue: Hoechst 33342 stain
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GRP465) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GRP465) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 .
The WB analysis of Vimentin antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 .
The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 .
The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 .
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GRP465) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of Vimentin antibody was published by Tsai CH and colleagues in the journal Sci Rep in 2017.PMID: 28195146
The WB analysis of Vimentin antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494
The IHC-P analysis of Vimentin antibody was published by Otti GR and colleagues in the journal PLoS One in 2014.PMID: 25397403
The WB analysis of Vimentin antibody was published by Chiu JH and colleagues in the journal J Transl Med in 2017.PMID: 28472954
The WB analysis of Vimentin antibody was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
The WB analysis of Vimentin antibody was published by Chiu JH and colleagues in the journal J Transl Med in 2017.PMID: 28472954
The WB analysis of Vimentin antibody was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (GRP465) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tu
Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 ?g of Vimentin antibody (GRP465).Western blot analysis was performed using Vimentin antibody (GRP465).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GRP465 as capture antibody at concentration of 5 μg/mL and as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to d
Sandwich ELISA detection of recombinant full-length Vimentin protein using GRP465 as capture antibody at concentration of 5 μg/mL and as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to d
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