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Product Overview
| Product Name | Ubiquitin antibody [GT751] |
|---|---|
| Catalog Number | GRP170 |
| Species/Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | The immunogen used to generate this antibody corresponds to Ubiquitin. |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Purity | Affinity purified by Protein G. |
| Clone ID | GT751 |
Product Description
Ubiquitin antibody [GT751]
Application Notes
| Dilution Range | WB: 1:500-1:20000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Ubiquitin antibody [GT751] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human endometrial carcinoma.Ubiquitin stained by Ubiquitin antibody [GT751] (GRP622) diluted at 1:200.
Ubiquitin antibody [GT751] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Ubiquitin stained by Ubiquitin antibody [GT751] (GRP622) diluted at 1:200.
Untreated (–) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT751] (GRP622) diluted at 1:500.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT751] (GRP622) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
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