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Product Overview
| Product Name | TNF alpha antibody |
|---|---|
| Catalog Number | GRP45 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Bovine |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Full length human TNF alpha Recombinant protein. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.04 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P01375 |
| Entrez | 7124 |
Product Description
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect
TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibod
TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TNF alpha antibody (GRP497) diluted at 1:400.
TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TNF alpha antibody (GRP497) diluted at 1:400.
TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma . TNF alpha antibody (GRP497) diluted at 1:500.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:500. The HRP-conjugated anti-rabbit Ig
TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GRP497) diluted at 1:500.Blue: Hoechs
TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. TNF alpha antibody (GRP497) dilution: 1:500.
TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GRP497) diluted at 1:500.Blue: Hoechst 3
TNF alpha antibody detects TNF alpha protein by western blot analysis.A. 30 μg NCI-H929 whole cell lysate/extract12% SDS-PAGETNF alpha antibody (GRP497) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibod
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pr
The WB analysis of TNF alpha antibody was published by Lee AS and colleagues in the journal Cardiovasc Diabetol in 2014.PMID: 24666525
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