TET1 antibody [N3C1]

Catalog Number: GRP63

Product Overview

Product Name	TET1 antibody [N3C1]
Catalog Number	GRP63
Species/Host	Rabbit
Reactivity	Human, Mouse, Monkey
Conjugation	Unconjugated
Tested applications	ChIP, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Alternative Names	(click to expand) tet methylcytosine dioxygenase 1 \| CXXC6 \| LCX \| bA119F7.1

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	3.66 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	Q8NFU7
Entrez	80312

Product Description

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GRP515) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (GRP515) diluted at 1:250.

TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (GRP515) dilution: 1:1000.

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GRP515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 stained by TET1 antibody [N3C1] (GRP515) diluted at 1:1000.Blue: Hoec

TET1 antibody [N3C1] detects TET1 protein by western blot analysis.A. 30 μg 293T whole cell lysate/extractB. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5% SDS-PAGETET1 antibody [N3C1] (GRP515) dilution: 1:5000 The HRP-

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GRP515) antibody at 1:1000 dilution.

Reviews

Write Your Own Review

You're reviewing:TET1 antibody [N3C1] - GRP63

Your Rating

1 star 2 stars 3 stars 4 stars 5 stars

Name

Institute

Application

Review