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SOX2 antibody [N1C3]

Catalog Number: GRP105

Product Overview

Product Name	SOX2 antibody [N1C3]
Catalog Number	GRP105
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	FACS, ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
Alternative Names	(click to expand) SRY-box 2 \| ANOP3 \| MCOPS3

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.15 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P48431
Entrez	6657

Product Description

Application Notes

Dilution Range	WB: 1:5000-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:100-1:500

Validation Images

SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500.

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GRP557) antibody at 1:80 dilution.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GRP557) dilution: 1:500.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.

SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used

SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Blue: Fluoroshield with DAPI.

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GRP557) antibody at 1:160 dilution.

Flow cytometry on human embryonic stem cells, staining with SOX2 (GRP557)antibody at 1:100 dilution(purple) or rabbit IgG (black).

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluo

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GRP557) antibody at 1:100 dilution.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:500.Red: phalloidin, a cytoskeleton

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GRP557) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

The ICC/IF analysis of SOX2 antibody [N1C3] was published by Chang WF and colleagues in the journal PLoS One in 2016.PMID: 27802323

The WB analysis of SOX2 antibody [N1C3] was published by Misuno K and colleagues in the journal Stem Cell Res Ther in 2013.PMID: 24423398

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] (GRP557).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GRP557) diluted at 1:200. The signal was developed using goat anti-rabbit

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557) or SOX2 antibody [GT1876] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] diluted at 1:500.EasyBlot anti-Rabbit IgG was

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker,

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