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Product Overview
| Product Name | SNAI1 antibody |
|---|---|
| Catalog Number | GRP154 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | O95863 |
| Entrez | 6615 |
Product Description
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GRP606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary an
SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNAI1 protein stained by SNAI1 antibody (GRP606) diluted at 1:1000.Blue: Hoechst 33342
SNAI1 antibody detects SNAI1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SNAI1 antibody (GRP606) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GRP606) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was dev
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GRP606) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
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