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Sheep anti-Rabbit IgG (H&L), HRP conjugated
Catalog Number: GRP12577
Product Overview
| Product Name | Sheep anti-Rabbit IgG (H&L), HRP conjugated |
|---|---|
| Catalog Number | GRP12577 |
| Species/Host | Sheep |
| Immunogen | Purified rabbit IgG (H&L) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified sheep IgG |
Application Notes
| Dilution Range | This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB) |
|---|---|
| Application Notes | Additional Information: Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins. Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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