SARS-CoV-2 trimeric spike protein (wt)

Size

50 ug (1 mg/ml)

Modifications

C-terminal transmembrane region replaced with a trimerization domain and a polyhistidine tag. Two stabilizing proline mutations and scrambled S1/S2 furin cleavage site (see reference).

Strain

SARS-CoV-2 Betacoronavirus

Isolate (Seq ID)

Wuhan-Hu-1 (GenBank: MN908947)

Expression System

CHOExpress^TM cells

Purity

> 90 % as determined by SDS-PAGE.

Conjugate

His

N-terminus

VNLT

Molecular Weight

The recombinant SARS-CoV-2 trimeric spike protein consists of 3576 amino acids with a predicted molecular mass of ~400 kDa.

Endotoxin

<1.0 EU per μg protein as determined by the LAL method.

Format

Liquid

Buffer

0.01M PBS, pH 7.4

Preservative

None

Storage

Store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Ship

Shipped on dry ice