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Rad50 antibody [13B3]

Catalog Number: GRP89

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Datasheet
Product Overview
Product Name Rad50 antibody [13B3]
Catalog Number GRP89
Species/Host Mouse
Reactivity Human, Mouse, Rat, Monkey
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Amino acids 1-425 of Rad50 expressed in E. coli.
Alternative Names (click to expand) Rad50 Double Strand Break Repair Protein | Nbsld | Rad502 | Hrad50 | Rad50
Product Properties
Form/Appearance Liquid: PBS
Concentration 1 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG1
Clonality Monoclonal
Purity Affinity purified by Protein G.
Clone ID 13B3
Uniprot ID Q92878
Entrez 10111
Product Description

RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions.

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000
Validation Images
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad50 protein stained by Rad50 antibody [13B3] (GRP541) diluted at 1:200.Red: phalloidin, a c
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad50 protein stained by Rad50 antibody [13B3] (GRP541) diluted at 1:200.Red: phalloidin, a c
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GRP541) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GRP541) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GRP541) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GRP541) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GRP541) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GRP541) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The WB analysis of Rad50 antibody [13B3] was published by Palagyi A and colleagues in the journal Mol Cancer in 2010 .
The WB analysis of Rad50 antibody [13B3] was published by Palagyi A and colleagues in the journal Mol Cancer in 2010 .
The WB, IP analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817
The WB, IP analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817
The WB analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817
The WB analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817
The WB analysis of Rad50 antibody [13B3] was published by Zhu J and colleagues in the journal EMBO Mol Med in 2013.PMID: 23341130
The WB analysis of Rad50 antibody [13B3] was published by Zhu J and colleagues in the journal EMBO Mol Med in 2013.PMID: 23341130
The WB analysis of Rad50 antibody [13B3] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663
The WB analysis of Rad50 antibody [13B3] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663
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