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Rabbit anti-Mouse IgG (H&L), ALP conjugated

Catalog Number: GRP12252

Product Overview
Product Name Rabbit anti-Mouse IgG (H&L), ALP conjugated
Catalog Number GRP12252
Species/Host Rabbit
Reactivity Mouse
Tested applications ELISA, ICC, WB, IHC
Immunogen Purified Mouse IgG, whole molecule.
Product Properties
Form/Appearance Liquid, clear, colorless.
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.Shelf life is one year from date of receipt.
Note For research use only.
Clonality Polyclonal
Purity Purified rabbit IgG
Application Notes
Dilution Range 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Application Notes Additional Information: Based on IEP, this antibody binds to: heavy chains on mouse IgGlight chains on all mouse immunoglobulinsNo reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis. ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative. Background: Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
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