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Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample

Catalog Number: GRP12250

Product Overview
Product Name Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample
Catalog Number GRP12250
Species/Host Rabbit
Reactivity Mouse
Predicted Reactivity Mouse IgG Heavy and Light chains (H&L)
Tested applications ELISA, WB, IHC
Immunogen Purified Mouse IgG, whole molecule
Product Properties
Form/Appearance Liquid
Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note For research use only.
Clonality Polyclonal
Purity Purified rabbit IgG
Application Notes
Dilution Range 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Application Notes Additional Information: No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Background: Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
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