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Rabbit anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse and rat serum

Catalog Number: GRP12609

Product Overview
Product Name Rabbit anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse and rat serum
Catalog Number GRP12609
Species/Host Rabbit
Reactivity Goat
Predicted Reactivity Goat IgG Heavy chains and Light chains on all Goat immunoglobulins
Tested applications ELISA, WB, IHC
Immunogen Purified Goat IgG, whole molecule
Product Properties
Form/Appearance Lyophilized
Storage Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note For research use only.
Clonality Polyclonal
Purity Purified goat IgG
Application Notes
Dilution Range 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Application Notes Additional Information: No reactivity is observed to non-immunoglobulin goat serum proteins. No reactivity is observed to serum from human, mouse and rat based on immunoelecrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Background: Rabbit anti-goat IgG is a secondary antibody conjugated to HRP which binds to goat IgGs in immunological assays. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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