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Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum
Catalog Number: GRP12347
Product Overview
Product Name | Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum |
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Catalog Number | GRP12347 |
Species/Host | Rabbit |
Product Properties
Form/Appearance | Lyophilized |
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Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Note | For research use only. |
Clonality | Polyclonal |
Purity | Affinity purified IgG |
Application Notes
Dilution Range | 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB) |
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Application Notes | Additional Information: This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis.Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Rabbit anti-goat IgG Fc,HRP conjugated, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase goat IgG. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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