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Rabbit anti-Goat IgG (H&L), ALP conjugated

Catalog Number: GRP12253

Product Overview
Product Name Rabbit anti-Goat IgG (H&L), ALP conjugated
Catalog Number GRP12253
Species/Host Rabbit
Reactivity Goat
Predicted Reactivity Goat IgG Heavy and Light chains (H&L)
Tested applications ELISA, ICC, WB, IHC
Immunogen Purified Goat IgG, whole molecule
Product Properties
Form/Appearance Liquid
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note For research use only.
Clonality Polyclonal
Purity Purified rabbit IgG
Application Notes
Dilution Range 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Application Notes Additional Information: No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative Background: Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.
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