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Rabbit anti-Fluorescein affinity purified, HRP Conjugated
Catalog Number: GRP986
Product Overview
| Product Name | Rabbit anti-Fluorescein affinity purified, HRP Conjugated |
|---|---|
| Catalog Number | GRP986 |
| Species/Host | Rabbit |
| Predicted Reactivity | No cross reactivity with immunoglobulin of any species |
| Conjugation | HRP |
| Immunogen | solid phase hapten-antibody capture |
Product Properties
| Storage | 2-8°C |
|---|---|
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Affinity purified |
Application Notes
| Application Notes | This product consists of affinity purified & absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3) by methods developed by The manufacturer. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free Enzyme is removed. Typical antibody concentration in this product is 1-2 mg/ml. Estimated HRP:IgG substitution is >1.5. This preparation is supplied in a Phosphate Saline Buffer stabilized with Bovine Serum Albumin (10 mg/ml) and preserved with 0.02% thimerosal. This product is suitable for immunoblotting, immunohistochemical and enzyme immunoassay applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and immunoblotting while 1 :200 - 1:1,000 is recommended for enzyme immunoassays. Recommended short term (< 6 months) storage is liquid at 2-8 C. For longer term storage, aliquot and freeze. NOTE: sodium azide is a potent inhibitor of peroxidase! Recommended chromogens are: Diaminobenzidine or TMB for immunohistochemistry; 4-chloro-napthol for immunoblotting and o-Phenylenediamine or ABTS for enzymeimmunoassay. |
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