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Product Overview
| Product Name | RAB5A antibody |
|---|---|
| Catalog Number | GRP127 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAB5A. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P20339 |
| Entrez | 5868 |
Product Description
RAB5A antibody
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Sample (50 μg of whole cell lysate) A: mouse brain 12% SDS PAGE GRP579 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RAB5A antibody detects RAB5A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. RAB5A antibody (GRP579) dilution: 1:500.
RAB5A antibody detects RAB5A protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract12 % SDS-PAGERAB5A antibody (GRP579) dilution: 1:1000
RAB5A antibody detects RAB5A protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g BCL-1 whole cell lysate/extractC. 30 ?g Raw264.7 whole cell lysate/extractD. 30 ?g C2C12 whole cell lysate/extract12 % SDS-PAGERAB5A antibody
Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody (GRP579) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary anti
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody (GRP579) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rab5A antibody immunoprecipitates Rab5A protein in IP experiments. IP Sample: 1 mg HeLa whole cell lysate/extract A. 30 ?g HeLa whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of Rab5A protein by 2 ?g of Ra
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