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Product Overview
| Product Name | PARP antibody |
|---|---|
| Catalog Number | GRP12 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ChIP, ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.3 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P09874 |
| Entrez | 142 |
Product Description
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
PARP antibody detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP protein stained by PARP antibody (GRP464) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker
PARP1 antibody detects PARP1 protein by western blot analysis.A. 30 μg HCT116 whole cell lysate/extract (untreated)B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr)7.5% SDS-PAGEPARP1 antibody (GRP464) dilution: 1:1000
PARP antibody detects PARP protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. PARP antibody (GRP464) diluted at 1:500.
Cross-linked ChIP was performed with Raji chromatin extract and 5 ?g of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GRP464) diluted at 1:2000.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GRP464) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GRP464) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary an
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GRP464) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
The WB analysis of PARP antibody was published by Li CJ and colleagues in the journal Evid Based Complement Alternat Med in 2012.PMID: 23091557
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