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p53 antibody

Catalog Number: GRP30

Product Overview
Product Name p53 antibody
Catalog Number GRP30
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ChIP, ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P04637
Entrez 7157
Product Description

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GRP482) antibody at 1:500 dilution.
p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr A. 5 ?g preimmune rabbit IgGB. 5 ?g of p53 antibody (GRP482)The precipitated DNA was detected by PC
p53 antibody detects p53 protein at nucleus by immunofluorescent analysis. Samples: HCT 116 cells mock (left) and treated with 30 ?M Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody
Sample (30 μg of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 μM cisplatin treatment for 24 hr 10% SDS PAGE GRP482 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the
p53 antibody detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.p53 stained by p53 antibody (GRP482) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p53 antibody detects p53 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 stained by p53 antibody (GRP482) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at
Sample (30 μg of whole cell lysate) A: A431 (GRP482) 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GRP482)) Input, 20ug of HCT116 lysates. The precipitated pr
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