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Product Overview
| Product Name | OAT antibody [N1C3] |
|---|---|
| Catalog Number | GRP115 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OAT. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.28 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P04181 |
| Entrez | 4942 |
Product Description
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme is the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:1000-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GRP567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/o/a/oat-antibody-n1c3_grp567_wb_3_2.jpg)
![OAT antibody [N1C3] detects OAT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: OAT protein stained by OAT antibody [N1C3] (GRP567) diluted at 1:500.Blue: Hoechst 33342 staining.](https://www.grp-ak.de/media/catalog/product/o/a/oat-antibody-n1c3_grp567_if_1_2.jpg)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GRP567) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect](https://www.grp-ak.de/media/catalog/product/o/a/oat-antibody-n1c3_grp567_wb_2_2.jpg)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GRP567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
OAT antibody [N1C3] detects OAT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: OAT protein stained by OAT antibody [N1C3] (GRP567) diluted at 1:500.Blue: Hoechst 33342 staining.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GRP567) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using OAT(GRP567) antibody at 1:100 dilution.
Sample (50 μg of whole cell lysate) A: Mouse brain 10% SDS PAGE GRP567 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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