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Product Overview
| Product Name | NPEPPS antibody [N3C2-2], Internal |
|---|---|
| Catalog Number | GRP147 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.68 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P55786 |
| Entrez | 9520 |
Product Description
This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000 |
|---|
Validation Images
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GRP599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/n/p/npepps-antibody-n3c2-2-internal_grp599_wb_1_2.jpg)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPEPPS(GRP599) antibody at 1:200 dilution.
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP599 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GRP599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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