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Product Overview
| Product Name | N-RAS antibody |
|---|---|
| Catalog Number | GRP42 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | WB |
| Immunogen | Full length human N-Ras Recombinant protein. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.41 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P01111 |
| Entrez | 4893 |
Product Description
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000 |
|---|
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was de
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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