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N-RAS antibody

Catalog Number: GRP42

Product Overview
Product Name N-RAS antibody
Catalog Number GRP42
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications WB
Immunogen Full length human N-Ras Recombinant protein.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 1.41 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P01111
Entrez 4893
Product Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was de
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GRP494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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