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Monoamine Oxidase B antibody [N2C3]

Catalog Number: GRP118

Product Overview
Product Name Monoamine Oxidase B antibody [N2C3]
Catalog Number GRP118
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ELISA, ICC, IF, IHC-P, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.88 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P27338
Entrez 4129
Product Description

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,ELISA: 1:1000-1:10000
Validation Images
Sample (50 μg of whole cell lysate) A: Mouse liver 7.5% SDS PAGE GRP570 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Monoamine Oxidase B antibody [N2C3] (GRP570) diluted at 1:500.
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GRP5
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GRP570) diluted at 1:500.Antigen
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GRP570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELISA detection of MAOB using GRP570 for capture at a concentration of 2.5 μg/mL and for detection at a concentration of 0.5 μg/mL.
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