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Product Overview
| Product Name | MMP2 antibody |
|---|---|
| Catalog Number | GRP34 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.37 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P08253 |
| Entrez | 4313 |
Product Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Immunofluorescence analysis of methanol-fixed HeLa, using MMP2(GRP486) antibody at 1:200 dilution.
MMP2 antibody detects MMP2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MMP2 antibody (GRP486) dilution: 1:500.
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP486 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MMP2 antibody detects MMP2 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MMP2 antibody (GRP486) dilution: 1:500.
MMP2 antibody detects MMP2 protein at extracellular matrix in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MMP2 antibody (GRP486) diluted at 1:500.
MMP2 antibody detects MMP2 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MMP2 antibody (GRP486) dilution: 1:500.
MMP2 antibody detects MMP2 protein at extracellular matrix in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. MMP2 antibody (GRP486) diluted at 1:500.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of MMP2 antibody was published by Gao Y and colleagues in the journal Sci Rep in 2016.PMID: 26813495
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation of MMP2 protein from U87-MG whole cell extracts using 5 ?g of MMP2 antibody (GRP486).Western blot analysis was performed using MMP2 antibody (GRP486).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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