MAFG antibody

Catalog Number: GRP143

Product Overview

Product Name	MAFG antibody
Catalog Number	GRP143
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ChIP, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.
Alternative Names	(click to expand) MAF bZIP transcription factor G \| hMAF

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.53 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	O15525
Entrez	4097

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500

Validation Images

MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAFG protein stained by MAFG antibody (GRP595) diluted at 1:500.Red: phalloidin, a cytoskeleton marker

MAFG antibody detects MAFG protein by western blot analysis.A.50 μg rat muscle lysate/extract 12% SDS-PAGEMAFG antibody (GRP595) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MAFG antibody detects MAFG protein by western blot analysis.A.50 μg mouse muscle lysate/extract 12% SDS-PAGEMAFG antibody (GRP595) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG(GRP595) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody (GRP595) diluted at 1:1000.

Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ?g of MAFG antibody (GRP595) .Western blot analysis was performed using MAFG antibody (GRP595) diluted at 1:500.EasyBlot anti-Mouse IgG was used as a secondary reagent.

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