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Product Overview
| Product Name | LOXL2 antibody |
|---|---|
| Catalog Number | GRP36 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.17 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q9Y4K0 |
| Entrez | 4017 |
Product Description
This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: LOXL2 protein stained by LOXL2 antibody (GRP488) diluted at 1:500..Blue: Hoechst 33342 staining.Sca
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GRP488) diluted at 1:5000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GRP488) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LOXL2 stained by LOXL2 antibody (GRP488) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 ?m.
LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse ovary.LOXL2 stained by LOXL2 antibody (GRP488) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.LOXL2 stained by LOXL2 antibody (GRP488) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GRP488) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The ICC/IF analysis of LOXL2 antibody was published by Wang M and colleagues in the journal J Exp Clin Cancer Res in 2017 .
The IHC-P analysis of LOXL2 antibody was published by Wang M and colleagues in the journal J Exp Clin Cancer Res in 2017 .
The WB analysis of LOXL2 antibody was published by Wang M and colleagues in the journal J Exp Clin Cancer Res in 2017 .
LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.LOXL2 stained by LOXL2 antibody (GRP488) diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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