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Product Overview
| Product Name | LDB1 antibody [N2C3] |
|---|---|
| Catalog Number | GRP116 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q86U70 |
| Entrez | 8861 |
Product Description
Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
![LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 antibody [N2C3] (GRP568) dilution: 1:500.](https://www.grp-ak.de/media/catalog/product/l/d/ldb1-antibody-n2c3_grp568_ihc_3_2.jpg)
![LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 antibody [N2C3] (GRP568) dilution: 1:500.](https://www.grp-ak.de/media/catalog/product/l/d/ldb1-antibody-n2c3_grp568_ihc_2_2.jpg)
![LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LDB1 protein stained by LDB1 antibody [N2C3] (GRP568) diluted at 1:500.Blue: Hoechst 33342 stai](https://www.grp-ak.de/media/catalog/product/l/d/ldb1-antibody-n2c3_grp568_if_1_2.jpg)
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 antibody [N2C3] (GRP568) dilution: 1:500.
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 antibody [N2C3] (GRP568) dilution: 1:500.
Sample (50 μg of whole cell lysate) A: Mouse brain 10% SDS PAGE GRP568 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LDB1 protein stained by LDB1 antibody [N2C3] (GRP568) diluted at 1:500.Blue: Hoechst 33342 stai
Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GRP568) antibody at 1:100 dilution.
LDB1 antibody detects LDB1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody (GRP568) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody wa
LDB1antibody immunoprecipitates LDB1 protein in IP experiments.IP Sample: 1000 μg 293T whole cell lysate/extract A. 20 ?g 293T whole cell lysate/extractB. Control with 2.5 ?g of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2.5 ?g of LD
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