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Product Overview
| Product Name | LC3B antibody [GT3612] |
|---|---|
| Catalog Number | GRP81 |
| Species/Host | Mouse |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 0.43 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Purity | Affinity purified by Protein A. |
| Clone ID | GT3612 |
| Uniprot ID | Q9GZQ8 |
| Entrez | 81631 |
Product Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000 |
|---|
Validation Images
![LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: LC3B protein stained](https://www.grp-ak.de/media/catalog/product/l/c/lc3b-antibody-gt3612_grp533_if_1_2.jpg)
![Untreated (–) and treated (+) HeLa whole cell extracts (50 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.](https://www.grp-ak.de/media/catalog/product/l/c/lc3b-antibody-gt3612_grp533_wb_3_2.jpg)
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.](https://www.grp-ak.de/media/catalog/product/l/c/lc3b-antibody-gt3612_grp533_wb_2_2.jpg)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.](https://www.grp-ak.de/media/catalog/product/l/c/lc3b-antibody-gt3612_grp533_wb_1_2.jpg)
LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: LC3B protein stained
Untreated (–) and treated (+) HeLa whole cell extracts (50 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GRP533) diluted at 1:500.
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