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Product Overview
| Product Name | KLF5 antibody |
|---|---|
| Catalog Number | GRP33 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.42 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q13887 |
| Entrez | 688 |
Product Description
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KLF5 protein stained by KLF5 antibody (GRP485) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GRP485) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the p
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GRP485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary ant
KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. KLF5 antibody (GRP485) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GRP485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The WB analysis of KLF5 antibody was published by Zhang J and colleagues in the journal Biosci Rep in 2014.PMID: 25205373
The WB analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The WB, IP analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The WB analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The IHC-P analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The WB analysis of KLF5 antibody was published by Chen HL and colleagues in the journal Mediators Inflamm in 2014.PMID: 25197166
The WB analysis of KLF5 antibody was published by Zhang J and colleagues in the journal Biosci Rep in 2014.PMID: 25205373
The WB analysis of KLF5 antibody was published by Zhang J and colleagues in the journal Biosci Rep in 2014.PMID: 25205373
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