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Product Overview
| Product Name | KLF4 antibody |
|---|---|
| Catalog Number | GRP23 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | FACS, ICC, IF, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.23 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | O43474 |
| Entrez | 9314 |
Product Description
Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Application Notes
| Dilution Range | WB: 1:500-1:3000,FACS: 1:50-1:200,IP: 1:100-1:500 |
|---|
Validation Images
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GRP475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (20 μg of whole cell lysate) A: mouse ESC 12% SDS PAGE GRP475 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: NIH-3T3 10% SDS PAGE GRP475 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GRP475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (20 μg of whole cell lysate) A: human ESC 10% SDS PAGE GRP475 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GRP475) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Flow cytometry on human embryonic stem cells, staining with KLF4 (GRP475)antibody at 1:100 dilution(purple) or rabbit IgG (black).
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GRP475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the p
The WB analysis of KLF4 antibody was published by Hao Z and colleagues in the journal Nat Commun in 2017 .
The WB analysis of KLF4 antibody was published by Hao Z and colleagues in the journal Nat Commun in 2017 .
Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 ?g of KLF4 antibody (GRP475).Western blot analysis was performed using KLF4 antibody (GRP475) diluted at 1:500.EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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