IGF1 antibody

Catalog Number: GRP95

Product Overview

Product Name	IGF1 antibody
Catalog Number	GRP95
Species/Host	Rabbit
Reactivity	Human, Mouse
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.
Alternative Names	(click to expand) insulin like growth factor 1 \| IGF \| IGF-I \| IGFI \| MGF

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	1.24 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P05019
Entrez	3479

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1 antibody (GRP547) diluted at 1:500.

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IGF1 protein stained by IGF1 antibody (GRP547) diluted at 1:500.Blue: Hoechst 33342

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GRP547) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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