HSP70 - Salmonid heat shock protein 70, Affinity purified

Catalog Number: GRP12192

Product Overview
Product Name HSP70 - Salmonid heat shock protein 70, Affinity purified
Catalog Number GRP12192
Species/Host Rabbit
Reactivity Fish
Predicted Reactivity Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)
Tested applications IP, WB, IHC
Immunogen KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.
Product Properties
Form/Appearance Lyophilized in PBS pH 7.4
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Note For research use only.
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
MW 70 kDa
Uniprot ID B5X4Z3
Application Notes
Dilution Range 5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)
Application Notes Additional Information: This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive. Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results) Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Reconstitution: For reconstitution add 200 µl of sterile water
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