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Product Overview
| Product Name | HPRT antibody |
|---|---|
| Catalog Number | GRP140 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.65 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P00492 |
| Entrez | 3251 |
Product Description
HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GRP592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. HPRT antibody (GRP592) diluted at 1:500.
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HPRT antibody (GRP592) diluted at 1:500.
HPRT antibody detects HPRT1 protein by western blot analysis.A. 50 μg mouse liver lysate/extract12% SDS-PAGEHPRT antibody (GRP592) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HPRT antibody (GRP592) diluted at 1:500.
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HPRT antibody (GRP592) diluted at 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(GRP592) antibody at 1:200 dilution.
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. HPRT antibody (GRP592) diluted at 1:500.
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