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Product Overview
| Product Name | HIF1 alpha antibody |
|---|---|
| Catalog Number | GRP65 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Bovine, Rabbit |
| Conjugation | Unconjugated |
| Tested applications | ChIP, ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.26 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q16665 |
| Entrez | 3091 |
Product Description
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:570 |
|---|
Validation Images
ChIP was performed with HepG2 chromatin extract and 5 ?g of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GRP517) dilution: 1:500.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha protein stained by HIF1 alpha antibody (GRP517) diluted at 1:200.Blue: Hoech
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GRP517) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pr
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis.A. 30 μg HepG2 whole cell lysate/extract (untreated) B.30 μg HepG2 whole cell lysate/extract ( 200 ?M CoCl2 treatment for 24 hr)C. 30 μg HepG2 whole cell lysate/extract (500
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GRP517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the prim
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GRP517) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detec
The WB analysis of HIF1 alpha antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 .
The WB analysis of HIF1 alpha antibody was published by Kudová J and colleagues in the journal PLoS One in 2016 .
The WB analysis of HIF1 alpha antibody was published by Demetriades C and colleagues in the journal Nat Commun in 2016.PMID: 26868506
HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha stained by HIF1 alpha antibody (GRP517) diluted at 1:500.Red:
The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
The WB analysis of HIF1 alpha antibody was published by Misuno K and colleagues in the journal Stem Cell Res Ther in 2013.PMID: 24423398
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
The WB, ICC/IF analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB, ICC/IF analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580
Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 ?M CoCl2 for 24 hr using 5 ?g of HIF1 alpha antibody (GRP517).Western blot analysis was performed using HIF1 alpha antibody (GRP517) diluted at 1:500.EasyBlot anti-R
HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.Samples: Untreated (left panel) and 500?M CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15
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