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GST-tag (rabbit antibody, polyclonal)
Catalog Number: GRP13166
Product Overview
| Product Name | GST-tag (rabbit antibody, polyclonal) |
|---|---|
| Catalog Number | GRP13166 |
| Species/Host | Rabbit |
| Predicted Reactivity | native and denatured forms of purified GST or GST fusion proteins |
| Tested applications | ELISA, WB |
| Immunogen | GST protein |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified using solid phase GST |
| MW | 26 kDa |
Application Notes
| Dilution Range | 1: 1: 1000 (ELISA), 1: 2000 (WB) |
|---|---|
| Application Notes | Additional Information: Overnight incubation of this antibody with dilution 1:5 000 with 0,5 µg of GST-tagged protein produced strong background but works fine with samples of total Escherichia coli lysate if GST-tagged protein is expressed in low amounts. Exact working dilution for each application needs to be determined experimentally. Background: GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags. Reconstitution: For reconstitution add 50 µl of sterile water. |
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