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GRK2 antibody [C2C3], C-term

Catalog Number: GRP107

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Datasheet
Product Overview
Product Name GRK2 antibody [C2C3], C-term
Catalog Number GRP107
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications FACS, ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.
Alternative Names (click to expand) G protein-coupled receptor kinase 2 | ADRBK1 | BARK1 | BETA-ARK1
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 1.04 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P25098
Entrez 156
Product Description

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GRP559) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GRP559) antibody at 1:500 dilution.
Sample (30 μg of whole cell lysate) A:NIH-3T37.5% SDS PAGE GRP559 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A:NIH-3T37.5% SDS PAGE GRP559 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GRP559) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GRP559) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample(30 μg whole cell lysate)A:MOLT4 (GRP559)7.5% SDS PAGE diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample(30 μg whole cell lysate)A:MOLT4 (GRP559)7.5% SDS PAGE diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 ?g of GRK2 antibody [C2C3], C-term (GRP559).Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GRP559).EasyBlot anti-Rabbit IgG was used as a secondary reage
Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 ?g of GRK2 antibody [C2C3], C-term (GRP559).Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GRP559).EasyBlot anti-Rabbit IgG was used as a secondary reage
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